摘要：Reproductive toxicity, with its many targets and mechanisms, is a complex area of toxicology;
thus, the screening and identification of reproductive toxicants is a main scientific challenge for the safety
assessment of chemicals, including the European Regulation on Chemicals (REACH). Regulatory agencies
recommend the implementation of the 3Rs principle (refinement, reduction, replacement) as well as
of intelligent testing strategies, through the development of in vitro methods and the use of mechanistic
information in the hazard identification and characterization steps of the risk assessment process. The EU
Integrated Project ReProTect (6th Framework Programme) implemented an array of in vitro tests to study
different building blocks of the mammalian reproductive cycle: methodological developments and results
on male and female germ cells, prostate and placenta are presented.
关键词：alternative methods, male and female germ cells, prostate, placenta, REACH.
