摘要：Problem statement: An automated software package is developed for quantifying Luteinizing Hormone (LH) release from pituitary gonadotropes in response to short pulses of Gonadotropin-Releasing Hormone (GnRH). Approach: These computer programs were designed to accommodate the release mechanism that consists of three stages, namely; (1) Production of different concentrations of effectors (such as receptor-hormone binds, G protein interactions, inositol 1,4,5-trisphosphate IP3), (2) Release of Ca2+ from the endoplasmic reticulum ER and finally (3) Release of LH through pumping of Ca2+ in and out of the cytosol. Results: The programs were coded in C++ computer language and implemented on Pentium PC. The designed model was intended to help in explaining the characteristics of LH release in response to various duration and different concentrations of GnRH pulses in the presence and absence of external Ca2+. Conclusion: Features of the short-term responses were simulated and fairly understood using receptor binding, dimerization, interaction of the dimerized receptor with a G protein, production of effectors that open voltage sensitive channels in cell membrane and finally open the Ca2+ channels in the ER and the Ca2+-dependent release of LH. Also, prediction of the varying concentrations of Ca2+ effects and comparison with experimental data was made possible.
关键词：Computer modeling and simulation; luteinizing hormone; G protein; endoplasmic reticulum; computer medical diagnosis
