Background/Aim. Burnout syndrome as a consequence of a long stress at workplace can seriously disturb health and quality of life in exposed workers. It is necessary to have adequate burnout prevention and its detection. Worldwide much attention is paid to protect burnout and methods for its determination constantly improve. In Serbia there has not been a study of that kind yet. The aim of the study was to investigate burnout syndrome impact on the quality of life of workers in food industry in Niš, and to call attention of researchers in Serbia on this phenomenon, as well as to test probability of applying the original, standardized questionnaires (CBI, ComQolA5) to working population in Serbia. Methods. This study was performed in Niš within a period from 2008 to 2009 in the Institute for Workers Health Protection. A total of 489 workers were included in this study by the use of the standard questionnaire for burnout (CBI) and quality of life (Com- QoL-A5). Scale confidence for measuring burnout and quality of life was determined by Cronbach α coefficient. ANOVA analysis was used for rating influence of burnout on the quality of life. Results. The values of Cronbach α coefficient showed a high confidence of the scale for measurement personal burnout (0.87), work-related burnout (0.86) and subjective quality of life (0.83). We detected increased scores as a result of personal burnout (60.0), as well as of work-related burnout (67.9). The workers suggested relationship with the family and friends as a very important part for their quality of life (10.8), health (9.8) and safety (8.0). Productivity (6.8), emotional well-being (6.6) and material property (4.5) had smaller influence on their quality of life. An increase in score of work-related burnout by 1 was statistically significantly related to decreasing inter scores for subjective quality of life in health (B = -0.097), relationship with family and friends (B = - 0.048), safety (B = -0.061) and place in community (B = - 0.105). A statistically significant relation between workrelated burnout and subjective quality of life in productivity, material and emotional well-being was not determined. Conclusion. There is a high score of both personal and work related burnout among manufacturing workers in food industry. A negative correlation between burnout and subjective quality of life was also proved impacting health and well-being of workers, but also their productivity. The questionnaires we used (CBI, ComQolA5) could be considered as reliable and valid instruments for testing burnout and quality of life in Serbia.