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  • 标题:Quantitative analysis of dendritic branching pattern of large neurons in human cerebellum
  • 本地全文:下载
  • 作者:Milošević Nebojša T. ; Ristanović Dušan ; Marić Dušica L.
  • 期刊名称:Vojnosanitetski pregled
  • 印刷版ISSN:0042-8450
  • 出版年度:2010
  • 卷号:67
  • 期号:9
  • 页码:712-716
  • DOI:10.2298/VSP1009712M
  • 出版社:Military Medical Academy, INI
  • 摘要:

    Background/Aim. Dentate nucleus (nucleus dentatus) is the most distant of the cerebellar nuclei and the major system for information transfer in the cerebellum. So far, dendritic branches of four different kinds of large neurons of dentate nucleus, have been considered mainly qualitatively with no quantification of their morphological features. The aim of the study was to test the qualitative hypothesis that the human dentate nucleus is composed of various types of the large neurons by quantitative analysis of their dendritic branching patterns. Methods. Series of horizontal sections of the dentate nuclei were taken from 15 adult human brains, free of diagnosed neurological disorders. The 189 Golgi-impregnated images of large neurons were recorded by a digital camera connected to a light microscope. Dendritic branching patterns of digitized neuronal images were analyzed by modified Sholl and fractal analyses. Results. The number of intersections (Nm), critical radius (rc) and fractal dimension (D) of dendritic branching pattern for four types of the large neurons were calculated, statistically evaluated and analyzed. The results show that there is a significant difference between four neuronal types in one morphometric parameter at least. Conclusion. The present study is the first attempt to analyze quantitatively the dendritic branching pattern of neurons from the dentate nucleus in the human. The hypothesis that the four types of the large neurons exist in this part of human cerebellum is successfully supported.

  • 关键词:cerebellum; cerebellar nuclei; anatomy; cross-sectional; dendrites; neurons
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