Background/Aim. Adipose tissue secrets a number of hormonal active substances that play an important role in the development of various metabolic diseases. The aim of this study was to determine the resistin significance in patients with various forms of metabolic diseases. Methods. The survey was conducted in a hospital in Laplje Selo, and included 102 patients suffering from diabetes mellitus, cardiovascular disease and renal insufficiency. All patients were verified and anthropometric characteristics and the basic hematological and biochemical analysis and resistin level were controlled. Results. The average age of the patients, 69 (67.6%) men and 33 (32.4%) women, was 55 ± 9.3 years. It was about two-thirds of smokers. The values of mean arterial blood pressure suggested that our patients had expressed hypertension, body mass index (28.5 ± 3.4 kg/m2) pointed to excessive well-fed, the circumference of hips (100 ± 10.8 cm), waist circumference (110 ± 17.4 cm) and the ratio of hip/waist circumference (1.05 ± 0.14) indicated a significant intra-abdominal distribution of fat tissue. More than one third of our patients had a certain form of cardiovascular diseases, renal failure was present almost in a fifth of patients, while diabetes mellitus was verified in 44.1% of the studied population. Significantly higher values of resistin concentrations were determined in patients with renal insufficiency and diabetes mellitus. In relation to the level of resistin, according to the number of metabolic diseases per patient, a statistically significant difference was achieved (F = 11.80, DF = 4.61, p < 0.001). Spearman correlation (r = 0.66, p < 0.001) found a statistically significant correlation between the number of diseases in one subject and the levels of resistin. Conclusion. In patients with diabetes mellitus and renal failure higher concentrations of resistin were recorded.