Lepenica river basin is axis of economic and urban development of Šumadija region. However, because of disorderly water regime of Lepenica river and its tributaries, it appears several hydrologic problems on this territory, as example insufficiency of drinking and irrigating water by one cite, and floods and torrents (especially in Kragujevac valley) by other cite. Particular problem is water quality and pollution in river basin. In this paper will be analyzed water quality of Lepenica river and artificial lakes, built in its river basin, according to the data of Republic Hydrometeorologic Institute of Serbia. Also, it will be present polluter cadastre in river basin.