Increased ultraviolet radiation potentially detrimental to health is a direct effect of the reduced ozone layer of the Earth’s atmosphere. Stratospheric ozone over the territory of Serbia and immediate surrounding depleted in the considered period of 1979-2008; its amount reduced by 7.6%. Solar and volcanic activities have significant effect on the interannual variability of the stratospheric ozone. Ozone layer over Serbia is 13.8% thicker than the planetary layer, with the ozone hole forming once in five years on average without serious health implications.