期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2012
卷号：4
期号：4
页码：207-212
出版社：MAXWELL Science Publication
摘要：The aim of this study was focused on isolation and purification of an iron binding peptide derived from shrimp processing by-products. The shrimp processing by-products was hydrolyzed by Flavourzyme to produce iron binding peptides hydrolysate. Then the hydrolysate with iron binding ability was isolated and purified by SPSepharose Fast Flow cation exchanger, Sephadex G-25 gel filtration and reversed phase high-performance liquid chromatography (RP-HPLC), respectively. A peptide of molecular mass 699 Da with strong iron binding ability was obtained and identified by mass spectrometry (MALDI-TOF-TOF) to be LPTGPKS after analysis and alignment in database. Results indicated that the production of iron-binding peptides from shrimp processing by-products supported further applications for high-value bioproducts in health care product.
