摘要：Microorganisms play a major role in solving various problems of the human needs in many ways. Invertase is one of the industrially valuable enzyme products, commonly obtaining from microorganisms. Invertases are intracellular as well as extracellular enzymes. The enzyme has wide range of commercial applications like, the production of confectionery with liquid. It also aids fermentation of cane molasses into ethanol. One of the best industrially applicable microorganism, is the beneficial yeast namely Saccharomyces cerevisiae. The organism has found its application in the field of brewing, baking, and also in medicine. The present study is focused on the isolation of the Saccharomyces cerevisiae from the sugar cane juice and comparison of its invertase activity along with that of the yeast strains got from the fermentation industries. The comparison was made with different parameters such as immobilized cells and the mutated cells. The study discloses the activity of invertase in relation to mutation and immobilization increased with respect to the time. Furthermore the invertase activity shows that the organisms subjected to mutation could be used for a large scale fermentation process on par with quick and efficient fermentation with relatively more productivity. These results showed for further analysis in the immobilization and or mutation mechanisms against S. cerevisiae for getting more metabolic product with low cost.in i
