摘要：Adult male and female frogs Rana ridibunda were exposed to 0.125x10-3mg Champion 50WP/g body weight administrated by intraperitoneal injection, 1 injection at 2 days in a scheme of 3 weeks. The animals were kept at 4-6°C, respectively at 22-24°C in tap water tank. At the end of the experiment we observe large quantities of Pearls reagent material in Kupffer cells, dilatation of blood vessels, peri-hepatocyte, peri-centrilobular, peri-sinusoidal and periportal fibrosis, an expansion of Disse spaces, presence of leukocyte infiltrates, vacuolated hepatocytes with small pyknotic nuclei, and necrotic areas in the parenchyma. Histological changes were more powerful at animals that were kept at 22- 24oC than 4-6oC. Under normal conditions, the largest amount of copper accumulates in the cytosol. When the amount of copper is high, it accumulates in organelles: nucleus and lysosomes. Copper accumulation in the lysosomes is associated with the initiation of detoxification process and is the beginning of its biliary excretion.tered t
