Spiritual care service is an indispensable part of the holistic health care approach. However a formal measure of its service quality remains lacking. This research therefore intends to, (1) identify the quality dimensions involved in spiritual care service, (2) understand the structure of customers in relation to the dimensions, and extend the research of service quality into spiritual care service by proposing a workable framework. This research conceptualizes spiritual care service quality as a second-order, five-dimensional construct that reflects consumers’ expectation on the service. This research presents the development and validation of a framework for the evaluation of the service. The five dimensions of respect, empathy, credibility, accompaniment and insightfulness can be seen as within a nomological network that illustrates varied demographical effects. The results are consistent to the multidimensional representation of service quality, offering insight into the relationships between each quality dimension and its structure of customers.