期刊名称：Potravinarstvo : Scientific Journal for Food Industry
印刷版ISSN：1338-0230
电子版ISSN：1337-0960
出版年度：2010
卷号：4
页码：308-316
出版社：Association HACCP Consulting
摘要：Changes in the endocrine system might be a useful indicator of lead (Pb) exposure and its potential toxicity in animals. The study aimed at examining expression of apoptosis-related (caspase-3) peptide in porcine ovarian granulosa cells from pregnant gilts after lead addition. Ovarian granulosa cells were incubated with/without lead acetate for 18 hours: group Max - 0.5 mg.ml-1 of lead acetate, group A - 0.25 mg.ml-1, group B - 0.083 mg.ml-1, group C - 0.063 mg.ml-1, group D - 0.046 mg.ml-1 and the control group - without Pb addition. Expression of caspase-3 was detected by immunocytochemistry. The expression of caspase-3 was influenced by Pb treatment. In our study, isolated ovarian granulosa cells were able to induce expression of apoptosis-related peptide after Pb administration at the doses of 0.5 mg.ml-1; 0.25 mg.ml-1; 0.083 mg.ml-1; and 0.046 mg.ml-1. Our results contribute to a knowledge of mechanisms of lead effect on ovarian granulosa cells of pregnant gilts. Obtained data indicate the interference of Pb in the pathway of apoptosis of porcine ovarian granulosa cells from pregnant gilts through intracellular substances such as caspase–3. This result contributes towards the understanding of mechanisms related to lead-induced alterations in porcine ovarian granulosa cells from pregnant gilts. This heavy metal (Pb) can be a potential risk factor during pregnancy of pigs.i
