标题：DETEKCIA A IDENTIFIKÁCIA DRUHU LACTOBACILLUS V GASTROINTESTINALNOM TRAKTE KURČIAT ZA POUŽITIA METÓD FISH A PCRDETECTION AND IDENTIFICATION OF LACTOBACILLUS SPECIES IN GASTROINTESTINAL TRACT OF CHICKEN BY FISH AND PCR ANALYSIS
期刊名称：Potravinarstvo : Scientific Journal for Food Industry
印刷版ISSN：1338-0230
电子版ISSN：1337-0960
出版年度：2010
卷号：4
页码：439-445
出版社：Association HACCP Consulting
摘要：The aim of the present study was a rapid detection and identification of Lactobacillus sp. in gastrointestinal tract of chickens by fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) analysis. As a biological material broiler chickens Hybro were used. They were fattening by the combined probiotic preparation (1st and 2nd experimental group) for elimination of pathogens and better utilization of feed. The 100 % of isolated strains covered Lactobacillus salivarius and Lactobacillus acidophilus. For quantification of bacterial count was used FISH method. The statistical evaluation showed significant differences (P≤0.001) between control group and first experimental group in the number of Lactobacillus sp. counts. The same differences were found between control group and second experimental group. In the number of Lactobacillus sp. counts were found statistical significant differences (P≤0.05) between first and second experimental group.
关键词：Lactobacillus sp.; chicken; identification; fluorescence in situ hybridization;polymerase chain reactionak
