标题：SLEDOVANIE EXPRESIE EXTRACELULÁRNEHO LEPTÍNOVÉHO RECEPTORA U VÝKRMOVÝCH KURČIAT ROSS 308MONITORING OF THE LEPTIN EXTRACELLULAR RECEPTOR EXPRESSION IN ROSS 308 BROILER CHICKENS
期刊名称：Potravinarstvo : Scientific Journal for Food Industry
印刷版ISSN：1338-0230
电子版ISSN：1337-0960
出版年度：2010
卷号：4
页码：474-478
出版社：Association HACCP Consulting
摘要：Leptin gene was identified in 1994 by positional cloning. Leptin is not only important in regulating food intake and energy balance, but also acts as metabolic and neuroendocrine hormone. The effect of this protein in relation to metabolism and food behavior of farm animals has not been adequately investigated. In farm animals, control of feed intake and obesity is economically important. The aim of our study was to optimize the methodological approach. We isolated total RNA from the tissues of heart, spleen, liver and abdominal fat. In the fat abdominal tissue RNA appears as a reduced, what could be caused by an alternative cutting of the molecule (splicing). In mammals, alternative splicing of leptin receptor produces several C-terminal isoforms, which are reduced. They have an important role in transport, cellular internalisation and degradation of leptin. Chicken leptin receptor is similar to its mammalian counterparts in terms of intron/exon structure. It is not known whether the chicken leptin receptor also undergoes by alternative splicing.
关键词：leptin receptor; OB-R alternative Splicing; bird; gene expression; following
