标题：DETEKCE ALEL PRO VYSOKOMOLEKULÁRNÍ PODJEDNOTKY GLUTENINŮ U TRITIKALE POMOCÍ DNA MARKERŮDETECTION OF ALLELES FOR HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNITS IN TRITICALE USING DNA MARKERS
期刊名称：Potravinarstvo : Scientific Journal for Food Industry
印刷版ISSN：1338-0230
电子版ISSN：1337-0960
出版年度：2010
卷号：4
页码：545-551
出版社：Association HACCP Consulting
摘要：Molecular markers were used to identify the allele composition of loci Glu-A1, Glu-B1 and Glu-D1 of high-molecular-weight (HMW) glutenin subunits in 15 triticale genotypes (varieties and breeding lines). Amplified DNA fragments of HMW glutenin alleles were separated by agarose slab-gel electrophoresis. Differences between all the three alleles at the locus Glu-A1 [Glu-A1a (encoding Ax1), 1b (Ax2*) and 1c (AxNull)] were observed. PCR reaction (annealing) of a specific marker was optimised for the allele Glu-A1b in PS3 primer combination. In the variety Palomino the alleles Glu-A1b and Glu-A1c were detected from different DNA isolations. DNA was isolated from a mixed sample and so it was not possible to distinguish whether it was an admixture in the seed sample or a heterozygous constitution of the genotype. Two alleles were detected at the locus Glu-B1 [Glu-B1-1a (encoding Bx7) and Glu-B1-1b (Bx7*)] by PS4 primer. The allele Glu-D1d (HMW subunits 5+10) was detected in breeding lines that carried translocations of segments of wheat chromosome 1D to rye chromosome 1R only. Identification of wheat Glu-1 alleles using DNA markers can be an alternative for early selection of triticale genotypes with good bread-making quality.onom
