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  • 标题:Clenbuterol Residues in Plasma and Urine Samples of Food-Producing Pigs During and After Subchronic Exposure to a Growth-Promoting Dose
  • 本地全文:下载
  • 作者:Pleadin, Jelka ; Gojmerac, Tihomira ; Bratoš, Igor
  • 期刊名称:Food Technology and Biotechnology
  • 印刷版ISSN:1330-9862
  • 电子版ISSN:1334-2606
  • 出版年度:2009
  • 卷号:47
  • 期号:1
  • 页码:67-74
  • 出版社:Sveuciliste u Zagrebu - Faculty of Food
  • 摘要:The aim of the study is to evaluate the suitability of plasma and urine as matrices for clenbuterol residue determination during and after its subchronic administration at a growth-promoting dose to male pigs, using previously validated enzyme-linked immunosorbent assay (ELISA) as a screening method and liquid chromatography tandem mass spectrometry (LC-MS/MS) as a confirmation method. A high correlation coefficient between these analytical methods was obtained for both urine (R=0.9800) and plasma (R=0.9970) concentrations. Study results show the plasma and urine concentration to vary greatly during oral treatment with clenbuterol for 28 days. The peak urine concentration ((88.54±50.54) ng/mL) recorded on day 21 was 40-fold peak plasma concentration ((2.25±1.54) ng/mL). After withdrawal period, the peak urine clenbuterol concentration ((42.93±10.52) ng/mL) recorded on day 0 was 24-fold plasma concentration ((1.79±0.97) ng/mL). The maximum allowed concentration of 0.5 ng/g in the liver as a regulated matrix for control of clenbuterol abuse was achieved in plasma on day 3 ((0.52±0.26) ng/mL) and in urine on day 7 of treatment withdrawal ((0.45±0.11) ng/mL). Study results indicate that urine and plasma may be suitable matrices for the control of clenbuterol abuse during fattening of food-producing pigs but have a limited value because of the rapidly decreasing concentration upon treatment withdrawal, in plasma in particular.
  • 关键词:clenbuterol residues; growth promoting dose; subchronic exposure; pig; urine; plasma
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