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  • 标题:Comparison of two commercial tests for quantitative analysis of histamines in fish
  • 本地全文:下载
  • 作者:Šimat, Vida
  • 期刊名称:MESO The first Croatian meat journal
  • 印刷版ISSN:1332-0025
  • 出版年度:2010
  • 卷号:XII
  • 期号:6
  • 页码:340-341
  • 出版社:ZADRUŽNA ŠTAMPA, d.d.
  • 摘要:Biogenic amines are known to cause intoxication or potentiate histamine food poisoning in people. Histamine is the most toxic biogenic amine formed in fish; therefore finding reliable and efficient methods to detect and quantify histamine and other biogenic amines in seafood early in the production chain is needed. Usefulness and efficiency of two test kits for quantitative analysis of histamine were evaluated in this paper as alternatives to the chromatographic methods if seafood production, when more advanced laboratory equipment is not available. The range of values obtained by histamine tests was similar in samples without the addition of standards, while in samples with standards it varied from -25 to 15% compared to the means. Recovery and repeatability of the test kits ranged from 98 to 114% and 7 - 14 % rsd. Both kits had standard curves with R2-values above 0.998 but were less efficient than the chromatographic method.
  • 关键词:histamine; ELISA; quantitative test; fish
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