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  • 标题:Determination of total bacterial count in raw milk by flow cytometry
  • 本地全文:下载
  • 作者:Samaržija, Dubravka ; Antunac, Neven ; Pogačić, Tomislav
  • 期刊名称:Mljekarstvo
  • 印刷版ISSN:0026-704X
  • 电子版ISSN:1846-4025
  • 出版年度:2004
  • 卷号:54
  • 期号:1
  • 页码:39-51
  • 出版社:Hrvatska Mljekarska Udruga
  • 摘要:The automatic flow cytometry as routine method for total bacterial count determination of raw ex-farm milk has recently been accepted in Croatia. This method significantly differs from the reference method (Standard Plate Count) mostly in the presentation of the results obtained. Therefore, this paper summarized experiences in the application of flow cytometry in the dairy laboratories practice. The principle and the practice of the method, methodological details and factors influencing the results were described. In order to avoid problems regarding the interpretation of the results, which are general problems of the quantitative microbiology, this article try to explain an appropriate conversion of the results with regards to SPC/ml, as an official method for the bacteriological quality proposal by the national legislation.
  • 关键词:total bacterial count; flow cytometry; accuracy of the method; reference method
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