期刊名称：Potravinarstvo : Scientific Journal for Food Industry
印刷版ISSN：1338-0230
电子版ISSN：1337-0960
出版年度：2010
卷号：4
期号：4
DOI：10.5219/78
出版社：Association HACCP Consulting
摘要：The aim of work was to optimalize the ELISA method to detect the adulteration of sheep milk and sheep milk products by cow milk in the laboratory. We have focused on laboratory testing of ELISA kit (β-Lactoglobulin ELISA Set, SEDIUM R&D) for detection of cow β-Lg in sheep milk order to obtain high-quality, reliable and economically advantageous method suitable for routine use in practice. The results shown that for the quality of adulteration determination it is necessary to verify the sensitivity of applied kit by the samples dilution in accordance with the producer declared quantification range contained in the manual ELISA kit. The starting point for obtaining of relevant data was to create separate regression curves with high determination coefficient, which allowed to quickly and easily detect the cow milk additions in sheep milk, cloddish sheep and Slovak sheep cheese.
