摘要：Bioprospection is the systematic search for sustainable and commercial uses of genetic and biochemical elements in biodiversity. Bioprospection of restriction enzymes (RE) of biotechnological interest is being done in Nicaragua since 1999. We searched for new restriction enzymes in bacteria from soils in unexplored environments such as volcanic and agricultural areas in the country. In this article we report the identification of isoschizomers for 14 known RE: BamHI, EcoRV, MboI, MaeI, HaeIII, EarI, BsoBI, BsaBI, Acl I, BsaWI, Nci I, BstNI, BssSI y HpaII, the last eight of them which are thermostable. The information on the RE found in Nicaragua was registered in REBASE (Restriction Enzyme Database). We highlight the importance of the study of microbial diversity in Nicaraguan volcanic environments because it advances research on the evolution of life, the extreme environments where it can be found, and its applications on biotechnology.
