摘要:Background: Brucellosis is an important re-emerging zoonosis with a worldwide distribution. Brucellosis in India is yet a very common but often neglected disease. Methods: A retro prospective study was done in western Rajasthan on PUO patients those who attended Hospital attached of Dr. SNMC Jodhpur. Total 570 samples were tested for Brucella antibodies titration. In study group samples of PUO Patients (420), Milkmen & Veterinarians (70), Meat Handlers (30) & Healthy Control (50) were taken for finding their Antibrucella antibody titers Typhoid by Widal Test, Malaria by MP Strip Test were included in exclusion criteria for PUO patients All samples were tested by Stained Febrile Antigen. Results: Positivity for Antibrucella Antibody was 25.72%, 26.66%, 37.14%& 6.00% in PUO Patients, Meat Handlers & Veterinarians, Milkman & Healthy Control respectively. Conclusion & Recommendations: As climatic conditions of Western Rajasthan mimics with Middle East where Brucellosis is prevalent, in clinical practice Brucellosis should be kept in differential diagnosis & management of PUO& all preventive measure should be used for prevention of this Zoonotic disease.. A safe and effective vaccine in human is not yet available. Prevention is dependent upon increasing public awareness through health education programmes and safe livestock practices. Active co-operation between health and veterinary services should be promoted.