Context: Animal bite, especially dog bite is an important public health problem in urban India. Socio-cultural practices and myths consider as major problem for post-exposure prophylaxis of animal bites. Objectives: To study the epidemiological characteristics and determinants of post-exposure prophylaxis of animal bite victims. Methodology: It was a cross-sectional study conducted among new cases of animal bites registered at Tertiary Care Centres of Surat city. Results: Out of total 382 cases of animal bites majority (58%) belongs to 15-45 years of age-group and 83 % were male. Stray dogs were involved in 94% animal bite cases. Majority (81%) of bites were unprovoked. Category II bites were seen in 204(54 %) of cases. In 81.4% cases lower extremities were affected. Only two hundred ninety two cases had attended the ARV clinic within 24 hours of bite. Only 75 % of cases had done the wound washing. Conclusion: Local wound treatment immediate after an animal bite is an important basic step in the management of any animal bite case and this was lacking in most of the subjects. Efforts to eliminate the stray dogs are required.