摘要：Beta-glucosidase gene of bacterian origin was cloned in Saccharomyces cerevisiae to enable growth on disaccharide, cellobiose. To promote the secretion of ß-glucosidase B, the catalitic domain of bglB gene was fused with the serine- threonine rich domain of the STA1 gene and was inserted into an yeast expression vector under control of the CYC- GAL inductible S. cerevisiae promoter. Expression of hybrid gene and proteine secretion was verified in Saccharomyces cerevisiae with p-nitrophenyl-ß-D-glucopyranoside as substrate. Genetically stable and regulated expression in Saccharomyces cerevisiae of ß-glucosidase activity is interesting for the development of strains able to ferment ß-glycosidic sugars (i.e. cellobiose and lactose).
