期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2013
卷号：5
期号：07
页码：839-842
出版社：MAXWELL Science Publication
摘要：The extraction of cellulase from commercial enzyme preparation by ammonium sulfate precipitation and its enzymatic characterization were studied. The results were as follows: the conditions of precipitation was 60% saturation of ammonium sulfate, the recovery rate of carboxymethylcellulase (CMCase) and Filter Paper enzyme Activity (FPA) were 73.8 and 71.4%, respectively. The enzyme has optimal temperature of 55°C, optimal pH of 4.8, the ions of Co2+, Mn2+ in the buffer lowered the activity of CMCase, but the Cu2+ in low concentration activated the CMCase. The ions of Co2+, Mn2+ and Al2+ in the buffer lowered the activity of FPA, but the Cu2+ and K+ in low concentration activated the FPA; Km and Vmax of the enzyme were 2.22×10-3 g/mL and 1.11×104 U/h using Lineweaver-Burk graphic method, respectively.
关键词：Carboxymethylcellulase; cellulase; filter paper activity; enzymatic property; salting out; ;
