文章基本信息
- 标题：Nucleoprotein filament formation is the structural basis for bacterial protein H-NS gene silencing
- 本地全文：下载
- 作者：Ci Ji Lim ; Sin Yi Lee ; Linda J. Kenney 等
- 期刊名称：Scientific Reports
- 电子版ISSN：2045-2322
- 出版年度：2012
- 卷号：2
- DOI：10.1038/srep00509
- 出版社：Springer Nature
- 摘要：
H-NS is an abundant nucleoid-associated protein in bacteria that globally silences genes, including horizontally-acquired genes related to pathogenesis. Although it has been shown that H-NS has multiple modes of DNA-binding, which mode is employed in gene silencing is still unclear. Here, we report that in H-NS mutants that are unable to silence genes, are unable to form a rigid H-NS nucleoprotein filament. These results indicate that the H-NS nucleoprotein filament is crucial for its gene silencing function, and serves as the fundamental structural basis for gene silencing by H-NS and likely other H-NS-like bacterial proteins.
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