This study examines the level of awareness of organizational culture (OC) of hotel managers in middle and upper level Hotel Units (HU) in Greece. A random sample of 140 hotel managers from middle and upper Hotel Units (HU) in Greece were surveyed using the Organizational Culture Assessment Questionnaire (OCAQ), a 30-item OC scale developed by Sashkin (1997). The results indicated that the mean values of the OC factors “managing change”, “achieving goals” and “cultural strength” are considered as average, while “coordinated teamwork”, “customer orientation” and the total OC score are considered to be high, when compared to Sashkin (1997) norms. Further results revealed significant differences only for the “customer orientation” factor. Generally, no significant differences were found among managers of middle and upper level HU for general awareness of OC. It is concluded that the results are rather homogenous, with all HU managers placing more or less the same importance to Sashkin’s five OC factors, with each HU manager reporting a rather satisfactory organizational culture.