摘要：The antioxidant activity of chitosan before micronization (BMC, average particle size of 1850 ± 26.3 μm) and after micronization (AMC, average particle size of 1.37 ± 0.2 μm) in grape juice was studied. Antioxidant activity was determined, including that of DPPH radicals, hydrogen peroxide and superoxide anion radicals, as well as ABTS radicals of BMC or AMC in grape juice. AMC exhibits stronger scavenging activity toward DPPH radicals, superoxide anion radicals and hydrogen peroxide than BMC. At a concentration of 1.0 mg/mL, AMC in grape juice exhibited 90.0%, 97.3% and 88.7% scavenging activities toward DPPH radicals, hydrogen peroxide and superoxide anion radicals, respectively. The TEAC (Trolox Equivalent Antioxidant Capacity) values of AMC (3.94 ± 0.19) greatly exceeded those of BMC (2.21 ± 0.10) in grape juice. The in vitro results in this investigation suggest the possibility that AMC can increase the antioxidant activity in grape juice. However, comprehensive studies must be performed to ascertain the in vivo safety of AMC in experimental animal models.
