摘要：In this study, we employed our optimized method of high performance liquid chromatography with electrochemical detection (HPLC-ED) to study the content of thiols. These are glutathions (reduced (GSH) and oxidized (GSSG) glutathione), which ratio is used as a marker of oxidative stress, and phytochelatins PC2, PC3, PC4 and PC5, which are very important for the protection of plant cells against adverse effects of heavy metals. Thiols content was studied in flax plants treated with various concentrations of cadmium(II) ions. Using our method, we were able to identify and quantify the levels of thiols, which varied depending on the applied dose of cadmium
关键词：flax; phytochelatins; high performance liquid chromatography with electrochemical detection (HPLC-ED).
