摘要：Background: Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals. O b jectives: We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure. Methods: We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C.assay with the comprehensive mammalian in vivo end point data from the ToxRef database. R esults: The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germline integrity by monitoring germline apoptosis and meiotic progression. C onclusions: This C.assay provides a comprehensive strategy for assessing environmental effects on germline function.
关键词：aneuploidy; C. elegans; chromosome segregation; germline;pesticides. Environ Health
