摘要：Introduction: Cystic fibrosis (CF) is inherited, metabolic, multisystem disease with various clinical symptoms. In the airways neutrophilic inflammation and increased levels of neutrophil elastase (NE) are observed even in yo ung children. The method of electrophoretic mobility of cell nuclei (EMN) is a way of assessment cell maturity. Observed distorted nuclei mobility is mostly dependent on chromatin distribution and remodeling. Purpose: To evaluate the EMN index in buccal cells collected fro m CF patients. Materials and methods:The stud y was conducted in the group of 15 CF patients aged 5-26 years. Results: In the healthy subjects we have observed that the lowest EMN index values were reach around 2 year of life (7.6 ¡À 3.1) and in the late senescence (8.5 ¡À 2.5) with the peak values around 16-21 years (61.4 ¡À 2.5). The results differed significantly fro m the healthy controls. Conclusion: EMN index in CF buccal cells may be a simple method to quickly assess the chromatin remodeling.
关键词：cystic fibrosis; children; electro-;phoretic mobility of cell nuclei
