The paper considers theoretical and applied foundations of the concept of the ecological corridors in nature conservation. Their relevance comes from recent ecological phenomenon of habitat fragmentation which is rapidly increasing during last decades. Habitat fragmentation is one of the main threats to richness and diversity of wildlife. Ecological corridors can mitigate the loss and fragmentation of habitat. Corridors perform as “bridges” between habitats for species and they provide a flow of the natural or even anthropogenic caused disturbances. In this paper we will present the meaning and significance of ecological corridors in nature conservation, as well as types of ecological corridors and their ecological benefits. Methodological and practical approaches in nature protection system in Serbia are included. [Projekat Ministarstva nauke Republike Srbije, br. 47007 i br. 176008]