出版社：Forestry Commission of the Hungarian Academy of Sciences
摘要：Pestalotiopsis funerea is a fungus reported mainly as a weak pathogen of conifers, although it has also been found causing severe damage on several hosts. The main aim of the study was to determine the optimal growth conditions in vitro for Spanish isolates of P. funerea and to evaluate the pathogenic effect of those isolates on Pinus pinea and Cupressus arizonica under field and lab conditions. Eight isolates of P. funerea derived from C. sempervirens, C. arizonica and Quercus pyrenaica were used in the assays. In the growth rate experiment, five culture media (PDA, MEA, WA, PCA and SCALA) and six temperatures (5, 10, 15, 20, 25 and 30 oC) were evaluated. In the pathogenicity tests, two different experiments were carried out: those in the lab consisted of inoculations in 30 mm long twigs of C. arizonica. In those of the field, twigs and needles of C. arizonica and P. pinea trees respectively were inoculated with mycelia by means of a wound. Four months after inoculations, twigs and needles were transported to the lab where necroses length was measured. The results presented here suggested that the P. funerea isolates from Spain had an optimum temperature for growth at 25 oC on SCALA media. The results also indicated that the fungus showed a virulence on C. arizonica and P. pinea after inoculating mycelia into a wound but host-preference was not observed. A certain correlation between growth rate in culture media and virulence was also observed on P. pinea needles
