期刊名称：Annals of the University Dunarea de Jos of Galati. Fascicle VI : Food Technology
印刷版ISSN：1843-5157
电子版ISSN：2068-259X
出版年度：2008
卷号：I
期号：XXXI
页码：17-22
出版社：Galati University Press
摘要：A semi-qualitative screening based on protease and amylase activity evaluation in a basal agar medium supplemented with insoluble chromogenic substrates based on AZCL (Azurine-Crosslinked with amylose or casein) using a plate assay was used for selecting the polar streptomycetes able to produce cold actives and alkaline amylases and proteases. This technique provides a specific and rapid simultaneous detection of high active hydrolase producing strains based on the visible solubilization of small particles of AZCL and the formation of haloes on plates. It has a great potential in increasing the efficacy of screening streptomycetes able to produce hydrolytic enzymes. This study revealed the potential of the selected streptomycetes isolated from polar soils to biosynthesize amylases and proteases cold-adapted at low temperatures (from 5 to 20oC) and alkaline pH values (8 to 9)
