期刊名称：Annals of the University Dunarea de Jos of Galati. Fascicle VI : Food Technology
印刷版ISSN：1843-5157
电子版ISSN：2068-259X
出版年度：2012
卷号：36
期号：2
页码：58-63
出版社：Galati University Press
摘要：The aim of the present research was to examinate the profile of intestinal microbiota of sterlet from theDanube River. Genomic DNAwas extracted from each gut fish and polymerase chain reaction (PCR) was used to amplify the conserved 16S ribosomal RNA gene. Using Denaturing Gradient Gel Electrophoresis(DGGE) of PCR amplified 16S rDNA to characterise the variability of bacterial populations, the results showed differentmicrobial profiles for 50% of fish. These results demonstrate the potential of PCR-DGGE-based analysis for identification of gut microbiota and could contribute to a better understanding of fish ecology
