摘要：This study was conducted to determine the toxicity effect of isolated Lactobacillus plantarum from cocoa bean fermentation on Sprague dawley rats. The toxicity effect of L. plantarum was tested using three types of doses which were 106cfu/mL (low dose), 108cfu/mL (medium dose), and 1010cfu/mL (high dose) and compared with a control group. Each dose is then formulated in dark chocolate and was toxicologically assessed. For sub acute toxicity test, rats were weighed, and their volume of water intake was recorded weekly which last for 28 days followed by haematology test at the final day of the experiment being conducted. To ascertain the survival of L. plantarum in the gastrointestinal tract of the rats, Feces Restoration Analysis was done by serial dilution with 10-1 to 10-6dilution factor on MRS agar. The sub-acute test showed neither toxicity effects nor death incidents occurred. There was no significant difference showed in any of the L. plantarum dosage blood parameter tests that were conducted at (p<0.05). For the platelet count, the rats treated with all dosages of L. plantarum for ALT, AST and ALP has a significant difference (p<0.05) when compared to control group (117 11.57 IU/L). There was no significant reduction in urea and creatinine value respectively. Both medium and high concentrations of L. plantarum vividly declined as compared to the control group (41-58%) and the low dosage group for haematocrit. In conclusion, the different dosages of L. plantarum in dark chocolate proven no toxicity effect and are safe for consumption
