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  • 标题:Utilization of liquid medium for rapid micropropagation of Stevia rebaudiana Bertoni
  • 本地全文:下载
  • 作者:M. Kalpana ; M. Anbazhagan ; V. Natarajan
  • 期刊名称:Journal of Ecobiotechnology
  • 电子版ISSN:2077-0464
  • 出版年度:2009
  • 卷号:1
  • 期号:1
  • 出版社:Society for Scientific Research
  • 摘要:Liquid shoot culture of Stevia rebaudiana Bertoni in MS medium containing BAP (1.5 mg/l) and IAA (0.5 mg/l) was developed and evaluated in relation to shoot multiplication, on average 37 new shoots per explants were obtained within 3 week. Shoot lets were regenerated from nodal explants of stevia through axillary shoot proliferation. For rooting different concentrations of IAA, IBA and NAA were used and highest rooting percentage (63%) was recorded on MS medium with IAA (1.0 mg/l). The rooted plantlets were hardened and successfully established.
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