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  • 标题:Comparison of three molecular sexing primers in a monomorphic tropical furnariid, the Spotted Barbtail (Premnoplex brunnescens)
  • 本地全文:下载
  • 作者:Jeff Port ; Harold F. Greeney
  • 期刊名称:Boletín SAO
  • 印刷版ISSN:0123-9082
  • 出版年度:2012
  • 卷号:21
  • 期号:1&2
  • 出版社:Sociedad Antioqueña de Ornitología (SAO)
  • 摘要:We examined the reliability of 3 previously identified molecular sexing primer sets for the identification of gender in the Spotted Barbtail (Premnoplex brunnescens).Primers developed by Griffiths et al. (1998) P2/P8, Kahn et al. (1998) 1237L/1272H, and Fridolfsson & Ellegren (1999)2550F/2718R were compared using blood samples taken from 30 adult individuals captured near Cosanga, Napo Prov., Ecuador.Comparison of genetic sexing results suggests that for the Spotted Barbtail, the Fridolfsson and Ellegren (1999) 2550F/2718Rand Griffiths et al. (1998) P2/P8 primers provided the most reliable results, although for most individuals, all three provided fragments suitable for sexing
  • 关键词:cloud forest; Furnariidae; molecular sexing; ;Premnoplex brunnescens;Spotted Barbtail
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