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  • 标题:Solid Culturing of Bacillus amyloliquefaciens for α-Amylase Production
  • 本地全文:下载
  • 作者:Gangadharan, Dhanya ; Sivaramakrishnan, Swetha ; Nampoothiri, Kesavan Madhavan
  • 期刊名称:Food Technology and Biotechnology
  • 印刷版ISSN:1330-9862
  • 电子版ISSN:1334-2606
  • 出版年度:2006
  • 卷号:44
  • 期号:2
  • 页码:269-274
  • 语种:English
  • 出版社:Sveuciliste u Zagrebu - Faculty of Food
  • 摘要:Fourteen different agroresidues were screened for alpha amylase production using Bacillus amyloliquefaciens ATCC 23842. Among them, wheat bran (WB) and groundnut oil cake (GOC) in mass ratio of 1:1 was proved as the best substrate source. Supplementation with 0.01 M KH2PO4 and 1 % soluble starch enhanced the enzyme yield considerably. Maximum enzyme recovery from the solid mass was obtained when extracted with 0.1 M acetate buffer, pH=5.0. Maximum enzyme titer expressed as units per mass of dry substrate obtained was 62 470 U/g after 72 hours of fermentation at 37 °C by using the above solid substrate mixture (5 g) with the initial moisture of 85 % and inoculated with Bacillus amyloliquefaciens of 2·109 CFU/mL.
  • 关键词:α-amylase; Bacillus amyloliquefaciens; solid-state fermentation; agroresidues
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