摘要:Extra- and intracellular tannase production by Aspergillus niger GH1 has been evaluated using submerged (SmF) and solid-state fermentation (SSF) at different temperatures (30, 40 and 50 °C). Effects of initial substrate (tannic acid) concentration, incubation time and temperature on tannase production in SSF have been studied. A. niger GH1 produced the highest tannase level (2291 U/L) in SSF at 30 °C during the first 20 h of culture at tannic acid concentration of 50 g/L, and under these conditions enzyme production was entirely extracellular. The decline in tannase activity after 20 h of incubation was associated with a concomitant increase in protease activity.