期刊名称：Pertanika Journal of Social Sciences & Humanities
印刷版ISSN：0128-7702
电子版ISSN：2231-8534
出版年度：2005
卷号：28
期号：1
出版社：Universiti Putra Malaysia
摘要：Eight previously designed polymorphic microsatellite loci, namely, Mnc434a, Mnc65b, Mnc441, MnBp5-l-02b, MnBp5-l-30b, MnBp5-2-02b, MnBp8-4-43a, and MnBp8-4-43b, were used to examine their modes of segregation in Mystus nemurus family study. Randomly selected broodstocks from Terengganu population were used to generate F1 population. DNA was isolated from tissue samples of the parents, whilst the whole one-month-old F2 progeny was used to isolate the DNA. Chi-square analysis was performed to test the significance of the results obtained. It was shown that all the eight microsatellite loci examined segregated close to Mendelian inheritance ratios, which would be useful in generating a genetic linkage map for this species.
