期刊名称：Pertanika Journal of Social Sciences & Humanities
印刷版ISSN：0128-7702
电子版ISSN：2231-8534
出版年度：1988
卷号：11
期号：3
出版社：Universiti Putra Malaysia
摘要：In this studyf the excised embryos of 11 recalcitrant species were studied to determine suitable media and cultural conditions for their development into whole plants. Murashige and Skoog's medium formulation modified slightly by the addition of 0.17 gl~l NaH2PO4 was found to be a suitable basal medium for all the species studied. The basal medium supplemented with 2 gl"1 activated charcoal and 1.0 mgl"1 NAA or IAA in combination with 1 mgl"1 Kinetin, BAP or 2iP was found to be suitable for all the 11 species where the embryos developed normally to form whole plants. For some of the species, soaking the sterile excised embryos in an antioxidant (10 mgl~l ascorbic acid and 5 mgl~^ citric acid) solution for 10-45 minutes prior to culture enhanced the normal development of embryos.
关键词：Embryo culture; recalcitrant species; media formulation
