文章基本信息
- 标题：Sirt1 activation by resveratrol is substrate sequence-selective
- 本地全文：下载
- 作者：Mahadevan Lakshminarasimhan ; David Rauh ; Mike Schutkowski 等
- 期刊名称：Aging
- 出版年度：2013
- 卷号：5
- 期号：3
- 页码：151-154
- 出版社：U.S.Department of Health & Human Service
- 摘要：
Sirtuins are protein deacetylases used as therapeutic targets. Pharmacological Sirt1 activation has been questioned since the in vitro activator resveratrol failed to stimulate deacetylation of several physiological substrates. We tested the influence of substrate sequence by analyzing resveratrol effects on Sirt1-dependent deacetylation of 6802 physiological acetylation sites using peptide microarrays. Resveratrol stimulated deacetylation of a small set of sites and inhibited deacetylation of another set, whereas most substrates were hardly affected. Solution assays confirmed these substrate categories, and statistical analysis revealed their sequence features. Our results reveal substrate sequence dependence for Sirt1 modulation and suggest substrates contributing to resveratrol effects.
-->SOME HEADER NAME-->Quisque ante ipsum, ullamcorper vel, eleifend sed, molestie sed, est. In scelerisque, elit semper ornare hendrerit, nisi nulla commodo sem,-->Quisque ante ipsum, ullamcorper vel, eleifend sed, molestie sed, est. In scelerisque, elit semper ornare hendrerit, nisi nulla commodo sem, Read more »-->Quisque ante ipsum, ullamcorper vel, eleifend sed, molestie sed, est. In scelerisque, elit semper ornare hendrerit, nisi nulla commodo sem-->--> - 关键词：resveratrol; Sirt1; Sirtuin; sequence dependent; activation; substrate specific modulation
