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标题：Age-related micro-RNA abundance in individual C. elegans
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作者：Mark Lucanic ; Jill Graham ; Gary Scott 等
期刊名称：Aging
出版年度：2013
卷号：5
期号：6
页码：394-411
出版社：U.S.Department of Health & Human Service
摘要：Non©\coding.small. RNAs.of.the. micro©\RNA.class.(miRNA).are. conserved.regulators. of. gene. function. with. a. broadimpact. on. biological. processes..We. screened. miRNA.levels. for. age©\related. changes. in. individual. worms. and. investigatedtheir. influence. on. the. lifespan. of. the. nematode. C.. elegans.. We. measured. the. abundance. of. 69. miRNAs. expressed. inindividual. animals. at. different. ages. with. over. thirty. five. thousand. discrete. quantitative. nano©\fluidic. polymerase. chainreactions.. We. found. that. miRNA. abundance. was. highly. variable. between. individual. worms. raised. under. identicalconditions.and. that. expression.variability.generally.increased.with.age..To.identify.expression.differences. associated.witheither.reproductive.or.somatic.tissues,.we.analyzed.wild.type.and.mutants.that.lacked.germlines..miRNAs.from.the.mir©\35©\41.cluster.increased.in.abundance.with.age.in.wild.type.animals,.but.were.nearly.absent.from.mutants.lacking.a.germline,suggesting.their.age©\related.increase.originates.from.the.germline..Most.miRNAs.with.age©\dependent.levels.did.not.have.amajor.effect.on.lifespan,.as.corresponding.deletion.mutants.exhibited.wild©\type.lifespans..The.major.exception.to.this.wasmir©\71,. which. increased. in. abundance. with. age. and. was. required. for. normal. longevity.. Our. genetic. characterizationindicates.that.mir©\71.acts.at.least.partly.in.parallel.to.insulin/IGF.like.signals.to.influence.lifespan
关键词：Aging;.miRNA;.DAF©\16;.Insulin/IGF;.mir©\71;.expression.profiling.