摘要：Bacillusstrains isolated from 'Iru' and 'Ugba' was screened for mannanase production and production condition wasoptimized. Twenty twoBacillusstrains isolated wereBacillus subtilisIE,Bacillus pumilusS5,Bacillus licheniformisS4,Bacillus cereusU9,Bacillus megateriumU1 andBacillus coagulansU3.Bacillus subtilisIE had the highestfrequency of occurrence (31.82%). After screening six strains were selected for mannanase production under optimizedcondition. pH 5.0was optimum for mannanase production byB. subtilisIE (0.1600 U/ml) andB. pumilusS5 (0.1989U/ml). Optimum growth was at pH 3 and 40oC for all the isolates exceptB. subtilisIE.B. subtilisIE (0.0700U/ml) andB. megateriumUI (1.561) had the highestproduction and growth. 30 and 36 hours incubation time wasoptimumformannanase production and growth.B. subtilisIE (0.203aU/ml) andB. megateriumUI (0.800a) had the highest.Agitation condition,fructose and glucose induced optimum mannanase production and growth, it ranged fro m 0.042–0.1179 U/ml and 0.074–0.611 in whichB. subtilisIE andB. licheniformisS4 had the highest. Urea and yeast extractwas the best nitrogen source for mannanase production and gro wth byB. subtilisIE (0.198 U/ml) andB. megateriumUI(1.561).
