The objective of this systematic review was to analyze the design, sample characteristics and methodological quality of scientific articles which used stretching programs. Material and method. The selection of the studies was based on the following criteria: (a) experimental, (b) the intervention should be based on the performance of systematic stretching routines; and (c) the flexibility assessment tools must measure the range of motion. Results: 34 articles were finally analyzed. Conclusions: The methodological quality of the studies ranged from 2 to 8 points, with an average of 5.2 points. Further studies are required to analyze the chronic effects of stretching on flexibility using: longitudinal repeated measures designs, populations with different levels of fitness and flexibility, and (c) blinder tester.