摘要:The pine shoot blight, canker, and collar rot pathogen Diplodia pinea can persist on or in asymptomatic red pine nursery seedlings, and it can proliferate after outplanting to cause disease and mortality. After lifting from nursery beds, seedlings are routinely kept in cold storage at nurseries. During and after shipment to customers, however, seedlings may be stored without refrigeration. In each of 2 years, we assayed seedlings from a bareroot nursery before and after storage for presence of this pathogen. Each trial included a storage treatment in which seedlings were kept at room temperature for 1 week after cold storage. Results demonstrated the effectiveness of nursery cultural practices and protective fungicide applications, as well as cold storage, to reduce the frequency of association of the pathogen with asymptomatic seedlings. We recommend that seedlings be kept in cold storage, even at moderately cool temperatures, before, and especially after, delivery to customers