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  • 标题:Streptomyces rimosus GDS(L) Lipase: Production, Heterologous Overexpression and Structure-Stability Relationship
  • 本地全文:下载
  • 作者:Vujaklija, Dušica ; Abramić, Marija ; Leščić, Ivana
  • 期刊名称:Food Technology and Biotechnology
  • 印刷版ISSN:1330-9862
  • 电子版ISSN:1334-2606
  • 出版年度:2003
  • 卷号:41
  • 期号:1
  • 页码:89-93
  • 语种:English
  • 出版社:Sveuciliste u Zagrebu - Faculty of Food
  • 摘要:Streptomyces rimosus lipase gene has been overexpressed in a heterologous host, S. lividans TK23. The maximal lipase activity was determined in the culture filtrates of the late stationary phase. Time course of lipase production was monitored by a modified plate assay. S. rimosus lipase gene has been located on the AseI B fragment approximately 2 Mb far from the left end of the S. rimosus linear chromosome. Out of eight examined streptomycetes, the presence of this rare type of bacterial lipase gene was detected in two belonging to the S. rimosus taxonomic cluster, and in one non-related species. Comparison of protein sequences of the Streptomyces lipolytic enzymes was performed. The result indicated the best structural stability of the putative S. coelicolor lipase-2.
  • 关键词:lipase; Streptomyces; plate assay; thermostability
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