期刊名称：Informationen aus der Fischereiforschung (Formerly Informationen für die Fischwirtschaft aus der Fischereiforschung)
印刷版ISSN：1860-9902
电子版ISSN：1861-2164
出版年度：2011
卷号：58
出版社：Bundesforschungsanstalt für Fischerei
摘要：In the last years farmed Pangasius (Tra-Pangasius, Pangasius hypophthalmus) from Vietnam has reached a considerable market share, whereas aquaculture of A sian Redtail Catfish (Hemibagrus wycki oides) is in it s infancy. Recently it has been detected by food control authorities in Hamburg, that Pangasius fillets have been mislabelled and sold as fillets produced from A sian Redtail catfish. The necessity to improve the analytical methods for differentiation of Pangasius and Redtail Catfish prompted us to evalu-ate the suitability of is oelectric focusing (IEF) and DNA -analysis for identification of the two species. IEF of water soluble proteins was found to be a fast, reliable and economical method for differentiation of raw fillets of Pangasius and Redtail Cat fish, as long as reference material is available.PCR-bas ed DNA analysis was performed as follows: (i) amplification of a 464 bp segment of the cytochrome b gene; (ii) se-quencing of the PCR product; (iii) comparison of the sequence with entries in GenBank using BLAST. The sequences of both species differed considerably, allowing the unequivocal differentiation between P. hypopht halmus and H. wyckioides
