期刊名称：Informationen aus der Fischereiforschung (Formerly Informationen für die Fischwirtschaft aus der Fischereiforschung)
印刷版ISSN：1860-9902
电子版ISSN：1861-2164
出版年度：2012
卷号：59
出版社：Bundesforschungsanstalt für Fischerei
摘要：To ensure the authentication of fishery products lacking biological characters, rapid species identification methods are required. Two DNA- and protein-based methods, PCR-SSCP (polymerase chain reaction - single strand conformation polymorphism) of a 464 bp segment of the cytochrome b – gene and isoelectric focusing (IEF) of water-soluble proteins from fish fillets, were applied to identify fillets of (sub-) tropical fish species available on the European market. Among the samples analysed were two taxonomically identified species from the family Sciaenidae and one from Sphyraenidae. By comparison of DNA- and protein patterns of different samples, information about intra-species variability of patterns, and homogeneity of batches (e.g. fillet blocks or bags) can be obtained. PCR-SSCP and IEF may be useful for pre-checking of a large number of samples by food control laboratories.
关键词：fish species identification; PCR; SSCP; IEF; Sciaenidae; Sphyraenidae
