Arbuscular mycorrhizal fungi (AMF) have the capacity to improve plant growth and its nutrition and allows for more efficient P absorption. The possible interaction between the AMF and nematodes suggests a new alternative in pathogen control, through the creation of nematode tolerance in plants inoculated with mycorrhizae. The objective of this project was to determine the effects of AMF on nutrient absorption in bananas (Musa AAA subgroup Cavendish clone Valery) and their relationship to the nematode Radopholus similis. The experiment was established as a completely random design with four treatments, two of them with different types of AMF (Glomus fasciculatum and a mixture of native mycorrhizae based on Glomus spp.), a treatment with Vydate, and a control. The AMF colonization on bananas was successful. The AMF treatments (G. fasciculatum and the mixture of native mycorrhizae) produced the same colonization, which averaged at 23.6 %. G. fasciculatum produced the highest pseudostem and the longest leaves on the banana seedlings. The AMF treatment produced a better P absorption (34.7 mg plant^-1). AMF effects on R. similis control were not found in any of the treatments; however, the short duration of the experiment should be considered.